WebDec 24, 2024 · Stimulation was performed using 50 ng/mL RANKL and 25 ng/mL MCS-F (both R&D Systems ® through Bio-Techne Ltd ® Abingdon, UK) in α-MEM medium supplemented with 25 mM HEPES for 10 days (Gibco™ through Thermo Fisher Scientific, Loughborough, UK). Media change was performed every 48 h. WebDec 7, 2024 · IL-6 in plasma was analyzed with Luminex Performance Human High Sensitivity Cytokine Panel (Biotechne, Abingdon, UK). FABP4 in plasma was analyzed with Human Premixed Multi-Analyte Kit (Biotechne).
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WebApr 11, 2024 · For the last three experimental groups, combinations of compounds were tested, i.e. AKU-005 + the specific CB1 antagonist and inverse agonist AM-251 (1 µM, Tocris Bioscience/Biotechne Abingdon, UK) (group 5), AEA + AM-251 (group 6), or AEA + the specific TRPV1 blocker capsazepine (20 µM, Tocris Bioscience) (group 7), were … WebSep 5, 2024 · The HEK293 TCF/LEF-GLuc cells with the highest signal-to-background ratio after challenging with rmWnt3a (Biotechne, Abingdon, UK) were grown at 37 °C in a 5% CO 2 95% humidified air incubator in DMEM, containing 10% FBS, 1% nonessential amino acids, 500 μg/mL G418. 4.3. Polymerase Chain Reaction how keith whitley die
Differential expression of functional chemokine receptors on …
Web(From Biotechne, Abingdon, UK) 143: ∗ (From Biotechne, Abingdon, UK) Open table in a new tab Multiplex immunoassay data analysis. Raw data was analyzed using xponent … WebJan 20, 2024 · The cells were then first stained using the baseclick™ EdU-Click 488 kit as described above, then stained using a rat-anti-BrDU antibody also recognizing CldU (BU1/75, Biotechne, Abingdon, UK), washed twice with PBS and stained with a goat-anti-rat Alexa594-tagged antibody (Abcam, Cambridge, UK). WebDec 14, 2024 · Abingdon Site Sales Office Lead Bio-Techne Aug 2024 - Jul 2024 1 year. Abingdon, England, United Kingdom Within this role I … how kefir is made